Part:BBa_K5071006
BGCII-1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 73
- 1000COMPATIBLE WITH RFC[1000]
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Composite part BBa_K5071006 (BGCII-1)
Name: BGCII-1
Base Pairs: 180 bp
Origin: Paceibacterales
Usage and Biology
BGCII-1 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
Cultivation
We used PCR to amplify the BGCII-1 gene, with a length of 180 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pACYCDuet-BGCII-gene143.
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